Development of a mariner-based transposon for use in Sorangium cellulosum.
نویسندگان
چکیده
In order to generate marked insertions in the myxobacterium Sorangium cellulosum, a transposon based on the eukaryotic mariner transposon was developed. The transposition frequency was increased with the use of a mutated tnp gene. The transposon randomly inserts into the chromosome, as demonstrated by targeted mutagenesis of the epoK gene.
منابع مشابه
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 69 10 شماره
صفحات -
تاریخ انتشار 2003